Upon the Pseudo- Diphtheria , etc. 43 



I/5ffler bacilli granules blue, bacilli brown. 

 Pseudo-diphtheria bacilli granules blue (if any are 

 present), bacilli brown. 



2. Koch-Bhrlich gentian violet, one minute, wash in 



water ; 10 per cent acetic acid, one minute, wash 



in water. 



IxJffler bacilli granules violet, bacilli decolorized. 

 Pseudo-diphtheria bacilli granules violet (if any are 



present), bacilli decolorized. 



3. Watery solution of dahlia, one minute. 



lyoffler bacilli granules deeply stained, bacilli light 



violet. 

 Pseudo-diphtheria bacilli bacilli deeply stained, 



clear space in centre. 



4. Watery solution of methyl violet, one minute. 

 IvofHer bacilli granules deep violet, bacilli less 



deeply. 



Pseudo-diphtheria bacilli bacilli deep violet, homo- 

 geneous. 



5. Watery solution of crystal violet, one minute. 

 Same results as with methyl violet. 



6. Watery solution of crystal violet, one minute, wash 



in water ; 10 per cent solution of iodin in water, 

 one minute, wash in water. 



Same results as with watery solution alone, except 

 that the pseudo-diphtheria bacilli show the seg- 

 mentation. 



From the results obtained in the study of the biological 

 characters of the different cultures it is evident that we 

 are dealing with two distinct groups of organisms ; cultures 

 20, 26, 27, 29 and 31, from their growth on agar, blood 

 serum and alkali-peptone bouillon, belong to the group of 

 so-called pseudo-diphtheria bacilli, while all the other cul- 

 tures belong to the group of xerosis bacilli, except culture 

 13, which I have not classified definitely, though it probably 



