Upon the Pseudo- Diphtheria, etc. 47 



An attempt was made to render a number of animals 

 immune to the several cultures by inoculating them at 

 intervals of four to ten days with cultures of the same 

 organism, and then after such treatment for six weeks to 

 two months they were inoculated with cultures of the 

 Loffler bacillus. The results obtained do not indicate that 

 any degree of immunity had been acquired from any of 

 the cultures employed. When fatal doses of the Loffler 

 bacillus were inoculated subsequently, they died from ex- 

 perimental diphtheria. 



The following are the results of the autopsies on the 

 animals which died in consequence of inoculations of the 

 different cultures, in the attempt to increase the virulence 

 by passing them through successive cultures of alkali-pep- 

 tone bouillon, along with the results of sections of the 

 organs of these animals : 



Autopsy on guinea pig No. 7. Died nine and a half 

 days after inoculation with i c. c. of culture 13. Not 

 greatly emaciated ; inguinal and axillary glands appear 

 slightly enlarged. Abdominal walls at site of inoculation are 

 slightly hyperaemic. Liver seems somewhat hyperaemic. 

 Spleen is enlarged and dark. Kidneys are slightly con- 

 gested. Adrenals are large, pale and brittle. The lungs 

 are congested and show large areas here and there that are 

 completely hepatized. Cultures made from the hepatized 

 lung show [the micrococcus tetragenous. Cultures made 

 from the other organs remained sterile. On section the 

 liver, kidneys and spleen showed more or less congestion, 

 aside from which nothing abnormal was noted. The lung 

 showed extensive infiltration into the air cells, with some 

 proliferation of the connective-tissue cells, and marked con- 

 gestion. 



Guinea pig No. 55. Died twenty-two hours after re- 

 ceiving a second inoculation of culture 27 (4 c. c.) into the 

 peritoneal cavity. The subcutaneous lymphatics were some- 

 what congested. There was a large amount of sero-sangui- 



