Upon the Pseudo- Diphtheria, etc. 49 



nucleus, or a fragmented nucleus ; others show fairly well 

 in outline. The lung shows here and there small areas of 

 cellular infiltration. Aside from the congestion, nothing 

 abnormal was noted in the kidneys. 



Guinea pig No. 65. Died ten days after being inocu- 

 lated with 4 milligrams of a 24-hour-old blood-serum cul- 

 ture of 20. The liver and kidneys showed considerable 

 congestion. The bladder was very much distended with 

 pale, straw-colored urine. In the interior of the bladder 

 was what appeared to be an old pseudo-membranous cast 

 of the lining membrane. It was whitish in color, soft, 

 friable, and stained imperfectly, the stained matter looking 

 like cell debris. A few bacilli were found in this matter. 

 The mucous membrane of the bladder was hyperaemic. 

 Numerous bacilli were found in cover-slip preparations 

 made from the seat of inoculation. Cultures made from 

 the internal wall of the bladder and from the pseudo-mem- 

 brane in the bladder showed the pseudo-diphtheria bacillus. 



Guinea pig No. 76. Had been inoculated at intervals 

 for about two months, five in number, with alkali-peptone 

 bouillon cultures of 26, with the idea of rendering it im- 

 mune. The animal died about thirty hours after the last 

 inoculation into the peritoneal cavity. On autopsy a small 

 hemorrhagic point was observed in the abdominal wall, at 

 the point of puncture with the needle, in which the bacilli 

 were found. There was a large amount of sanguinous 

 fluid in the peritoneal cavity. Adhesions of the abdomi- 

 nal organs and recent peritonitis were also noted. The 

 organs were congested ; the adrenals were slightly hyper- 

 aemic. The pericardial sac also contained a little clear 

 fluid. Cover-slips made from the peritoneal fluid showed 

 leucocytes filled with bacilli. Cultures from the exudate in 

 the peritoneal cavity showed the pseudo-diphtheria bacillus. 



Guinea pig No. 98. Was inoculated subcutaneously 

 with 4 c. c. of a 48-hour-old alkali-peptone bouillon culture 

 of 27, and died eighteen days later. On autopsy no lesion 



