50 Comparative Studies 



was found at the site of inoculation. There was no fluid 

 in the internal cavities. Spleen, kidneys and liver were 

 normal ; adrenals slightly hyperaemic. The lungs showed 

 areas of inflammation. Cover-slips made from the lungs 

 showed micrococci. On section, the liver, kidneys, spleen 

 and adrenals were found normal. The lung was found to 

 be very much conjested, with infiltration of cellular ele- 

 ments, showing areas of complete consolidation. 



It will be observed that the results obtained in the autop- 

 sies on these animals are somewhat variable. On the whole, 

 the lesion's observed were dissimilar from those found in ex- 

 perimental diphtheria. In several of the animals some slight 

 subcutaneous edema was noted, but nothing very marked. 

 There was in no instance distinct glandular enlargement, 

 either of the external lymphatic glands or of the retro- 

 peritoneal glands, as noted usually in inoculations with the 

 Loffler bacillus. The appearance of the adrenals, while at 

 times somewhat hyperaemic, was usually the contrary 

 that is, they were rather pale and anaemic. 



Sections of the organs of these animals presented no dis- 

 tinctive features. Usually, death supervened after two or 

 more inoculations with the same organism, at intervals of 

 five to seven days. The most common feature in these 

 autopsies was the apparent lowered vitality of the animal, 

 as the result of the inoculations ; and in several instances 

 the animal evidently succumbed to secondary inflamma- 

 tion, more particularly inflammation of the lung, as the 

 result of the invasion of pathogenic cocci. 



The results of the inoculation experiments afford no as- 

 sistance in classifying these organisms. Neither do the 

 results of the growth in the different culture media present 

 features which are uniformly constant with regard to the 

 different cultures. However, from their biological charac- 

 ters, as noted more particularly on agar-agar and blood 

 serum with regard to the macroscopic appearance of the 

 cultures, we may classify them into two groups, more or less 



