THE BACTERIOLOGY OF THE OYSTER AND OTHER SHELLFISH 



Experiment VI. 



45 



At time of mixing ... 

 After 172 hours 



Experiment VII. 



At time of mixing ... 



After 504 hours (water kept at 8° C— 10° C.) 



Experiment VIII. 



At time of mixing .. 

 After 504 hours 



325 

 2 



325 

 79 



325 

 o 



III. — The Infection of the Oyster with the B. typhosus, and its 



Removal by Washing. 



These experiments were undertaken to ascertain whether oysters offered a favour- 

 able nidus for the multiplication of typhoid bacilli. 



Healthy oysters were placed in large glass vessels containing sea-water, to which 

 was added surface scrapings from agar plates previously inoculated with the typhoid 

 bacillus. 



By means of a syringe the water was aerated once or twice daily. . 



The contents of the stomach were pipetted out in the manner that will be 

 indicated further on, and agar plates made ; at the same time the water in which the 

 oyster was placed was likewise analysed. 



Table showing number of Organisms present in Stomach after infecting water. 



The above table shows that the typhoid bacillus does not increase in the body or in 

 the tissues of the oyster. The figures would rather indicate, comparing the large number 

 of bacilli present in the water with those found in the alimentary tract, that the bacilli 

 perish in the intestine. We showed their presence up to ten days, and Dr. Klein up to two 

 or three weeks. 



