rr.ate-ace tic mixture made by addinf; five per cent, 

 glacial acetic to a saturated aqueous solution of 

 Hp;C1 . This v/as often used hot. The material vms 

 cleared in xylol and imbedded in parafine. Sec - 

 tions were cut from five to fifteen mikrons in 

 thickness. For staining a combination of Haemalun 

 and Bismark Brown was tried, but ^lemming's Triple 

 was found to be more satisfactory and was used al- 

 most exclusively. It was necessary before the young 

 carpels could be sectioned in parafine to carefully 

 and laboriously remove the hairs from their bases, 



which 



on account of their thick walls^could not be cut in 

 parafine, but would invariably tear the sections. 

 To avoid this necessity Celloidin imbedding was us- 

 ed in a few instances, but it was impossible to get 

 the sections thin enough for most purposes by this 



