17 



Table #23. Pat Antigen and Anti-coli Serum. 



Table #24. Pat Antigen and Antiprotein Serum 



Table #25. Fat Antigen and Normal Serum 



+ = Complete precipitation 



* = Partial " 



- = No " 



The results are very indicative of a specific pre- 

 cipitatin reaction, in which the fats play the most 

 important part. Too much weight, however, should not be 

 attached to these reactions of precipitins because in 

 some cases (preliminary titrations) spontaneous reaction 

 occurred. Such reactions also frequently occurred after 

 the fifteen minute incubation period. This was probably 

 due to the unstable dispersoid phase of the antigen, so 

 that it was very susceptible to the reactions of the 

 various sera. 



D, Anaphylactic Reactions. It was found by trial 

 that 1.0 cc. of a 1:10 dilution of the fatty antigen was 

 usually fatal to guinea pigs when injected directly into 

 the heart, but the pigs could withstand the same amount 

 of a 1:20 dilution. This amount, therefore, was used as 

 the "toxic" dose. The animals were sensitized by intra- 

 cardiac injections of 1.0 cc. of a 1:500 dilution. They 

 promptly recovered from the effects of the injections and 

 gradually gained in weight. Tv/elve days after the sensi- 

 tizing dose the pigs received the "toxic" dose. The 

 results are recorded as follows: 



