324: Granule Cells of Paneth in Intestinal Glands of Mammals 



lamina of which separates it more or less completely from its neighbors. 

 The granules stain intensely in iron hematoxylin and in neutral gentian 

 but remain quite unaffected by muchaematein or mucicarmine. The 

 proximal or basal zone of the cell contains an oval nucleus which is 

 surrounded by a small quantity of protoplasm which takes a slightly 

 deeper stain than that of neighboring cylindrical cells. Some of the 

 cells contain a larger quantity of this basal protoplasm and in a few of 

 the cells this exhibits a distinct radial striation, in which case the deeper 

 stain is largely confined to the striae. The presence of these basal stria- 



FIG. 3. Bottom of gland of Lieberkuhn of the guinea-pig after twenty-four- 

 hour fast. From a preparation stained by orange-rubin-toluidene blue 

 method. Leitz 1/12, Oc. 4. 



tions was, however, more easily demonstrated in those animals which 

 were protected from an excess of physiological stimulation by controlling 

 the amount of food taken and supplying it at regular intervals. Fig. 3 

 represents the lower end of the gland from the small intestine of a guinea 

 pig which had fasted for twenty-four hours after receiving a mixed meal 

 of carrots and oats. The aspect of the cells in this case is very different 

 from that seen in the animal feeding irregularly. In the first place the 

 granules are more than twice as large, and there appears to be a larger 

 number, although, for obvious reasons, it is difficult to be sure of this. 

 In sections of the intestine stained with toluidene blue alone, or with 



