76 POISONOUS PROTEINS 



when the second vaccine is used on a fresh ani- 

 mal, fever appears and continues for three 

 days. When a highly virulent anthrax bacillus 

 is employed there may be no fever and death 

 follows within six or seven hours. Gamaleia 

 made similar observations in other infections 

 and came to the following conclusion : ' ' Fever 

 is not a result of bacterial growth, but on the 

 contrary is consequent upon a reaction on the 

 part of the body against the bacteria and leads 

 to their destruction." Furthermore he found 

 that nonpathogenic bacteria, living or dead, 

 led to the development of fever. I think that 

 these experiments, made more than a quarter 

 of a century ago, furnish strong support of my 

 theory that fever is due to the parenteral de- 

 struction of proteins. One year later this work 

 was confirmed by Charrin and Buffer and was 

 shown to hold good for nonbacterial proteins as 

 well. In 1890 Buchner induced the characteris- 

 tic phenomena of inflammation calor, rubor, 

 tumor, and dolor by the subcutaneous injec- 

 tion of diverse bacterial proteins. Krehl and 

 Matthes induced fever by the parenteral ad- 

 ministration of albumoses and peptones, but did 

 not obtain constant and uniform results, be- 

 cause as we now know they did not recognize 

 the necessity of regulating the size and fre- 



