PAET V. 



TISSUE CELLULAE PEOTEIN POISONS. 



Cummings and Chambers* have prepared the 

 protein poison from various organs and tis- 

 sues and have tested its effects upon both homo- 

 logous and heterologous animals. They de- 

 scribe their methods of preparing the tissue as 

 follows : Tissues including muscle, brain, heart, 

 lungs, liver, pancreas, and kidney were obtained 

 from the dog, goat, ox, and rabbit. Because 

 the protein poison can be obtained from blood 

 constituents it was necessary to remove these 

 before clotting occurred, thus securing organs 

 free from water soluble protein. The follow- 

 ing procedure was carried out to gain this re- 

 sult with the dog, goat, and rabbit : The ani- 

 mal was first anesthetized, then a glass cannula 

 was introduced into the abdominal aorta; the 

 jugulars were then cut and water was forced 

 under mild pressure into the circulatory sys- 

 tem. Several liters of wash water were used 

 in each case, the washing being continued until 



*Cummings and Chambers: Jour. Lab. and Clin. Med., i, 428. 



