106 POISONOUS PKOTEINS 



the fluid running from the jugulars had lost its 

 red tinge. The ox organs were removed im- 

 mediately after death, and each was washed 

 free from blood by forcing water through its 

 circulatory system. The organs were cut into 

 half-inch cubes and washed in water with fre- 

 quent changes ; the final wash water gave none 

 of the tests for protein. The tissues were then 

 dehydrated in 80 per cent and 100 per cent 

 alcohol each for twenty-four hours. They were 

 then dried and powdered with a fine meat 

 grinder. 



The tissue thus prepared was split into poi- 

 sonous and nonpoisonous parts by a 2 per cent 

 solution of sodium hydroxide in absolute alco- 

 hol. The poisonous products were tested out 

 on animals. Conclusions are stated as follows : 



1. Vaughan's protein poison can be prepared 

 from tissue cells of the exsanguinated organs 

 of multicellular animals. 



2. The tissue cellular protein poisons are not 

 only toxic for heterologous species, but also for 

 homologous species. 



3. The M.L.D. of the protein poisons here 

 reported for the guinea-pig and the rabbit is 

 in proportion to their relative body weights 

 when given by the intraperitoneal method of in- 

 jection; when given intravenously, however, it 



