I 



I have never be< - • 



. . - e to say, the egg of a 



; the first, fish 

 possibly the first vertebrate ich this opening was 



discovered. Whether this e -■ is transparent or not I can 

 not say, but in it Doye're ''43) foui i just over 

 Lisqui 1 if en "a its diameter as L/llc 



A. L Fertilizatio . 



jrs vary - r conclusions, or 

 .aore correctly their conjectures, as tc the time of fertiliz- 

 ation. A prior - ion to be 

 effected at the time of transfer. Pre e surest way 

 to determine the time of impregnation would be to take a male 

 immediately after the transfer, cut through the pouch just, 

 back of the forward end behind the genital opening, and then 

 Lne the eggs in the hinder part of t :h for spermat- 

 This I had intf . the past 

 jus transfers fish 

 kept in a - 1 , yet I saw the copulation on one 

 . only (i.i 1903.) between two pairs of fish. The se em- 

 it solute necessity for keej i se . for the • 

 es of se. . ::, prevent acrificing either to 

 determine this point. 



