I 



Artificial Fertilization, 

 - c e 

 This was tried bj the wet methoi and once 



The e sea 



in strained sea 

 lot of r . s careful 



drained, and over t s poured sperm from testes which had 



been torn up . Ctly dry dish. These were allowed 



to stand for a few minutes and were then placed in clean run- 

 ning sea water. The females were certainly ripu for spawn- 

 ing, and the males were well grown ^^ had not recently borne 

 eggs, so they were presumably fertile. A control experiment 



of his last lot in running sea 

 water wit out t ..: ad ition of sperms. In all cases the re- 

 sults were the same, Vt the end of one aid one-half hours, 

 protoplas] seen collecting aw 



two to t . . noticed t 



,-jned sli there was 



pretty clearly defined roun 



c of orange-red oil drops, ' of four 



to six hours, the germinal disc was at its prime, but nei. 

 then nor subsequent time was there any trace of seg- 



mentation. From this time on, the germinal disc gradually 

 lost its sharp outlines, flatten 



