vii OXIDATION-PRODUCTS 239 



Siegfried, 1 on hydrolysing oxyprot-sulphonic acid with HC1, obtained 

 bases which could be precipitated with phospho-tungstic acid. The 

 platinum salt had the formula C 8 H 22 N 2 3 Cl 6 Pt, while a silver salt 

 consisted of C 6 H 13 N 3 2 . NH0 3 . AgN0 3 . 



Subsequently Bondzynski and Zoja, 2 working under the direction 

 of Bunge, used purer material and obtained for oxyproteic- or oxy- 

 prot-sulphonic acid the following percentage figures : 



C H N S 



Crystallised egg- 

 albumin . 50-73 7-02 14-70 



Haemoglobin of 



the horse . 52'32 6'96 16'04 



Caseinogen (Ham- 



marsten) . ' 49'11-52'07 6'39-7'10 14'63-14'99 0'71-0'76 



Bernert, 3 Ehrmann, 4 and v. Furth 5 have continued the investiga- 

 tion of Maly's products in Hofmeister's Laboratory. 



Bernert has split up oxyprot-sulphonic, prepared from egg-albumin 

 by means of fractional precipitation with ammonium sulphate, into 

 two fractions, both of which gave negative results with the xantho- 

 proteic test and the reactions of Millon and Adamkiewicz. When 

 they are dissociated by means of HC1 they gave rise to leucin and 

 aspartic acid, but not to tyrosin. When fused with KOH, free fatty 

 acids and pyrrol, but neither indol nor skatol, were obtained. Maly's 

 view that oxyprot-sulphonic acid consists of albumin which has simply 

 become oxidised is disproved by the fact that after precipitating and 

 filtering off the oxyprot-sulphonic acid, Bernert found albumoses, 

 peptones, fatty acids and basic products in the filtrate. 



The sulphur seems to be in a peculiar state ; Maly and Bernert 

 failed to obtain the lead sulphide reaction, and assumed therefore that 

 the whole of the sulphur which is usually split off as the sulphide was 

 oxidised. Schulz 6 found, however, that oxyprot-sulphonic acid still 

 contains 0'33 per cent of detachable sulphur. This amount is less than 

 that usually split off, and therefore it would appear that that sulphur 

 which under ordinary conditions is most readily split off is exactly the 

 sulphur which is changed by oxidation, and hence the negative results 



1 Siegfried, Ber. d. deutsch. diem. Ges. 24. 418 (1891). 



2 St. Bondzynski and L. Zoja, Uber die Oxydation der Eiweisssto/e mit Kalium- 

 permanganat, Zeitschr. f. physiol. Chem. 19.225 (1894). 



3 J. Bernert, fiber Oxydation von JEiweiss mit Kaliumpermanganat, ibid. 26. 272 

 (1898). 



4 Ehrman, Uber die Peroxyprotsauren, Inaugural Dissertation, Strassburg (1903). 



5 0. v. Furth, Zeitschr. f. physiol. Chem. 44. 279 (1905). 



6 F. N. Schulz, ibid. 29. 86 (1899). 



