312 



CHEMISTRY OF THE PROTEIDS 



CHAP. 



EXPERIMENT 8c 



The sublimate and salt solutions were of the same strength as in 

 Experiment 8 B, the constants in this case being the salt and the 

 variables the sublimate and water. 



The immediate results : All solutions from A to M are opalescent ; 

 D to H show a flocculent precipitate after the first three shakes ; on 

 continuing to shake this precipitate disappears, but re-forms after five 

 minutes, though to a much lesser extent than at first. The specific 

 gravity of egg-white lies between / and K, nearer to /. / after ten 

 minutes shows traces of a flocculent precipitate, similar to the primary 

 one seen in D and H. K, L and M are milky opalescent with no trace 

 of flocculi ten minutes after the experiment. 



After forty-eight hours all the tubes from A to M are markedly 

 opalescent, the opalescence and the amount of precipitate in the bottom 

 of the test-tube increasing gradually from A to M. In M the upper 

 one -tenth of the solution is somewhat clearer than the subjacent 

 portion, showing that a second crop of flocculi is about to settle down. 

 K appears more milky than either I or L. M in general character 

 strongly resembles G of Experiment 8 A. 



General conclusions are given on the next page. 



EXPERIMENT 9 



A saturated solution of sublimate in 0*5 per cent sodium chloride 

 (Gaule's solution), and this solution diluted with an equal bulk of 

 water, were compared with a saturated solution of sublimate in 

 distilled water and also with this solution diluted with an equal bulk 

 of distilled water. 



