452 CHEMISTRY OF THE PEOTEIDS CHAP. 



fish contain, according to Miescher and Schmiedeberg, 1 96 per cent of 

 protamin-nucleate, the other albumins being only present in traces. 

 As the so-called 'chromatin' of the nuclei is basophil, i.e. is acid in 

 character, there is no doubt that the nuclear chromatin network 

 consists essentially of acid, nuclein-like substances (Miescher, 2 Ehrlich, a 

 Malfatti, 4 Kossel, 5 Zacharias, 6 Lilienfeld, 7 Heine, 8 and the author, 

 who has entered fully into the question of micro-chemical methods in 

 his book on Physiological Histology }. Q 



The inter-relation between nucleins and nucleic acids on the 

 one hand, and globulin-like bodies on the other hand, during cell- 

 metabolism has been especially investigated by Lily Huie, working 



ler the author's direction. 10 



Nucleo-proteids resemble many ferments as regards their solu- 

 bilities, and these two classes of compounds are therefore frequently 

 considered together. Hammarsten 11 obtained nucleo-proteid along with 

 trypsin from the pancreas ; Pekelharing, 12 Schumow-Simonowsky, ia 

 Nencki and Sieber u a nucleo-proteid besides pepsin from the gastric 

 mucous membrane and from the gastric juice ; Pekelharing 15 the 

 fibrin ferment as well as a nucleo-proteid from the blood and from 

 the thymus. This purely chemical result is in full agreement with 

 the histological evidence, for L. H. Huie, 16 working with the author, 



1 F. Miescher and 0. Schmiedeberg, Arch.f. exper. Path. u. Pharm. 37. 1 (1896). 



2 Miescher, 'Die Spermatozoen einiger Wirbelthiere, ' Verh. d. naturf. Gesellsch. 

 in Basel, 6. 138-208 (1874). 



3 Ehrlich, Farbenanalytische Untersuchungen z. Hist. u. Klinik d. Blutes, Gesammelte 

 Mitth. Berlin 1891, Hirschwald. 



4 Malfatti, Ber. d. naturw. med. Vereins in Innsbruck (1891-92). 



5 A. Kossel, Arch.f. (Anat. u.) Physiol 1891, p. 181. 



6 E. Zacharias, Ber. d. deutsch. botan. Ges. 11. 190 and 299 (1893) ; 14. 270 

 (1896) ; 16. 185 (1898) ; 19. 377 (1901) ; 20. 238 (1902). Verhandl. d. natur- 

 wissenschaftl. Vereins Hamburg, 1900 and 1901. See also L. Heine, Zeitschr. f. physiol. 

 Ghem. 21. 494 (1896). 



7 L. Lilienfeld, ibid. 1893, p. 391; 'Uber d. Wahlverwandtschaft d. Zellelemente 

 z. gewiss. Farbstoffen,' Arch. f. (Anat. u.} Physiol. 1903 ; 'Uber d. Farbenreaction d. 

 Mucins,' ibid. ; 'Zur Chemie d. Leucocyten, ' Zeitschr. f. physiol. Ghem. 18. (1894). 



8 Heine, 'Die Mikrochemie d. Mitose, zugleich erne Kritik microchemischer Methoden/ 

 ibid. 



9 Gustav Mann, Methods and Theory of Physiol. Hist. Clarendon Press, 1902. 



10 L. H. Huie, Quart. Journ. of Micros. Soc. 39. 387 (1896-97), and 42. 203 

 (1899). 



11 0. Hammarsten, Zeitschr. f. physiol. Ghem. 19. 19 (1893). 



12 C. A. Pekelharing, ibid. 22. 233 (1896) ; 35. 8 (1902). 



13 0. 0. Schumow-Simonowsky, Arch.f. exper. Path. u. Pharm. 33. 336 (1896). 



14 M. Nencki and N. Sieber, Zeitschr. f. physiol. Ghem. 32. 291 (1901). 



15 C. A. Pekelharing, Zentralbl. f. Physiol. 1895, p. 102 ; C. A. Pekelharing and 

 W. Huiskamp, ibid. 39. 22 (1903). 



16 L. H. Huie, Quart. Journ. of Micr. Soc. 39. 387 (1896-97), and 42. 203 (1899). 



