4:62 CHEMISTRY OF THE PROTEIDS CHAP. 



7. The Nudeo-Proteid of the Suprarenal Capsules 



Jones and Whipple l prepared and analysed it. It resembles the 

 nucleo-proteid of the pancreas. Gamgee and Jones 2 determined 



a D = +48-1. 

 Amongst its dissociation-products are guanin and adenin. 



8. The Nudeo-Proteid of the Liver 



This compound has been investigated by Wohlgemuth. 3 Fresh 

 minced ox-liver was repeatedly boiled out with water ; the combined 

 extracts treated with acetic acid to precipitate the proteids ; the 

 precipitate kept under alcohol, which was frequently changed and 

 extracted with ether to remove the fat ; dissolved in dilute sodium 

 carbonate and reprecipitated with dilute acetic acid. On redissolving 

 and reprecipitating, the phosphorus-content was found to be 3*05 

 per cent. The precipitate was preserved for some weeks under 

 alcohol and ether, and finally dried at 60. From 50 kilogrammes liver 

 were obtained 140 grammes of nucleo-proteid. Analysis gave the 

 following percentage-composition : 



045-22 H 5-72 N 16'67 S 0-637 P 3'06. 



Wohlgemuth calculates that 100 grammes of liver-nucleic acid 

 contain 



Xanthin . . . 2 '25 2 6 grammes. 



Guanin . . . 2 "5 402 



Adenin . . . 1'9051 



Hypoxanthin . . . 1'7875 



These figures, compared with those given by Steudel (see p. 441) for 

 the xan thin-bases of thymus-nucleic acid, show that the liver contains 

 more guanin and xanthin, but less adenin, while the hypoxanthin is 

 present in about equal amounts in the thymus and in the liver. 



From 95 grammes of nucleo-proteid Wohlgemuth obtained, accord- 

 ing to his last paper, 0*723 g. histidin (?), 1*08 g. lysin, and 3*38 g. 

 arginin. Other substances obtained were xanthin, hypoxanthin, guanin, 

 adenin, tyrosin, leucin, glycocoll, alanin, phenylalanin, a-prolin, 

 glutamic-, aspartic-, oxyamino-, oxydiamino-, sebacic acids, and /-xylose. 



1 W. Jones and G. H. Whipple, Amer. Journ. of Physiol. 7. 423 (1902). 



2 A. Gamgee and W. Jones, Hofmeister's Beitrage, 4. 10 (1903). 



3 J. Wohlgemuth, Zeitschr. f. physiol Ghem. 37. 475 (1903) ; 42. 519 (1904) ; and 

 44. 530 (1905). 



