x H^EMIN AND H^IMATIN 519 



anilin-molecules which may be introduced into hsemin, there is no 

 doubt that anilin is introduced, because the molecular weight of the 

 new compound is raised, and the percentage amount of iron is 

 lowered without any iron being split off. 



The compound resulting from the first interaction of hsemin and 

 anilin is acetone-insoluble, while the final products are acetone-soluble. 

 As all compounds are insoluble in caustic soda, it is assumed that 

 the hydroxyl groups of hsemin play a part in the reaction. 



Hetper and Marchlewski l prepared hsemin by means of propionic 

 acid, and obtained hsemin crystals which were somewhat larger than 



* acet-haemin ' crystals, but they differed in no other respect. 2 



The chloroform solutions of hsemin are made thus : Hsemin is 

 dissolved in a mixture of chloroform and quinine, and then a few drops 

 of acetic acid are added : 



Band a A655 630 

 ft A655 534 

 7 A524 497 



In concentrated solutions the bands ft and y fuse, and a feeble line is 

 seen at the Na-line. Very similar bands are obtained with the 

 dimethyl-ether of hsemin. Hsemin in chloroform + quinine, cinchonin, 



or ammonia, but without acetic acid, shows two bands : 







a A615 582 



ft A506 475 (ill-defined) 



Dimethyl-hsemin has the same spectrum. Addition of acetic acid 

 produces the three bands given above. Acetic acid and chloroform 

 solutions of hsemin and its dimethyl ethers in very dilute solutions 

 show a band on the Tl-line. By addition of quinine this band is 

 changed to the K/3 line. 3 Hetper and Marchlewski and Kiister agree 

 in that acet-hsemin may be prepared from Morner's hsemin, but while 

 Kiister found no OC 2 H 5 group, Hetper and Marchlewski found it in 

 traces. 



Hsematin 



Attention has already been drawn on p. 507 to the fact that the 

 dissociation of hsemoglobin into an albuminous fraction and into 

 the ferri-compound : hsematin, may be brought about by acids, or by 

 alkalies, or by heat, and that we have also to distinguish between 



* natural hsematin 'and 'artificial hsematin' (p. 517). 



1 J. Helper and L. Marchlewski, Zeitschr. f. physiol. Chem. 41. 38 (1904). 



2 Photographs in Bull, de I'Acad. des Sc. de Cracovie, Mai 1904. 



3 Cp. Gamgee, Zeitschr. f. Biol. 1896, p. 505. 



