110 



STUDIES ON FERMENTATION. 



mycoderma vini on the surface of saccharine liquids and in their 

 depths, unaccompanied by any other species, has the most 

 important bearing on the theory of alcoholic fermentation, we 

 may pursue it through a few examples with all the detail that 

 it allows of. 



On June 21st, 1872, we sowed some mycoderma vini in three 

 flasks, with double necks. A, B, C (Fig. 22), containing some 



Fig. 22. 



wort. The spores employed for the purpose were obtained from 

 plants growing on sweetened yeast- water in an ordinary closed 

 flask. This had been impregnated with spores from plants 

 grown on wort, which in turn had sprung from spores taken 

 directly from mycoderma vini growing on wine. 



The several impregnations were effected by means of a plati- 

 num wire, held by forceps, both having been first cleaned by 

 passing through flame, and then smeared with the fungoid 

 films. 



By this series of growings in closed vessels, which were but 

 momentarily open at the time when we dropped the spores into 

 them, we secured the separation of the mycoderma from all 

 foreign organisms ; and more particularly from germs of myco- 



