STUDIES ON FERMENTATION. 



241 



tripod above a gas flame, and in place of the vessel of mer- 

 cury substitute a porcelain dish, under which we can put a 

 gas flame, and which contains some fermentable, saccharine 

 liquid, similar to that with which the flask is filled. We boil 

 the liquid in the flask and that in the basin simultaneously, and 

 then let them cool down together, so that as the liquid in the 

 flask cools some of the liquid is sucked from the basin into the 

 flask. From a trial experiment which we conducted, determin- 

 ing the quantity of oxygen that remained in solution in the 

 liquid after cooling, according to M. Schiitzenberger's valuable 

 method, by means of hydrosulphite of soda,* we found that the 

 three litres in the flask, treated as we have described, contained 

 less than one milligramme (O'Olo grain) of oxygen. At the 

 same time we conducted another experiment, by way of com- 

 parison (Fig. 61). We took a flask, B, of larger capacity than 



Tig. 61. 



the former one, which we filled about half with the same 

 volume as before of a saccharine liquid of identically the same 

 composition. This liquid had been previously freed from 

 alterative germs by boiling. In the funnel surmounting A, we 

 put a few cubic centimetres of saccharine liquid in a state of 



* [NaH SO^iTiow called Sodium Jit/j^osuJphite. See p. 355, footnote. — D.C.E.] 



K 



