STUDIES ON FERMENTATION. 



285 



Into a flask, like that represented in Fig. 67, of 2'5 litres 

 (about four pints) in capacity, we put : — 

 Pure, crystallized, neutral tartrate of 



lime . . 

 Phosphate of ammonia 

 „ magnesium 



,, potassium 



Sulphate of ammonia. . 



(1 gramme =15-43 grains.) 

 To this we added pure distilled water, so as to entirely fill the 

 flask. 



In order to expel all the air dissolved in the water and 

 adhering to the solid suBstances, we first placed our flask in a 

 bath of chloride of calcium, in a large cjlindrical white iron 

 pot, set over a flame. The exit-tube of the flask was plunged 

 in a test-tube of Bohemian glass three-quarters full of distilled 

 water, and also heated by a flame. We boiled the liquids in 

 the flask and test-tube for a sufl&cient time to expel all the air 

 contained in them. We then withdrew the heat from under the 

 test-tube, and immediately afterwards covered the water which 

 it contained with a layer of oil, and then permitted the whole 

 apparatus to cool down. 



