STUDIES ON FERMENTATION. 375 



Total oxygen at first 



remainino: 



that has disappeared 

 in sohition 



in combination . . 



cc. 

 29-40 

 26-04 



3-36 

 0-54 



2-82 



Or per litre, 35*2 cc. (9"8 cub. ins. per gallon). 



The colour of the wort in this experiment had become sen- 

 sibly similar to that of the wort in the preceding experiment. 



Third Experiment. — In another experiment we left the flask, 

 for the same length of time again, after it had been refilled 

 wich air and reclosed, at a temperature which varied between 

 2° and 4° C. (35-6° and 39-2° F.). In this case we found- 

 Volume of air analyzed . . . . 27 '8 



After the action of potash . . . . . . 27*8 



After pyrogallic acid . . . . . . 22*3 



Oxygen . . . . . . . . b'5 



Composition of the gas : — Per cent. 



Oxygen . . . . . . . . . . 19'7 



Nitrogen 80-3 



cc. 



Total oxygen at first 29*40 



remainins: . . . . 27'58 



that has disappeared . . 1\S2 

 in solution . . . . 0'44 



,. „ in combination . . . . 1"38 



Or per litre, 17"20 cc (4'8 cub. ins. per gallon). 



In this last experiment the wort was scarcely darker in 



