376 STUDIES ON FEKMENTATION. 



colour. Its colour, compared with that of wort cooled on the 

 coolers in the brewery, was slightly darker ; but the difference, 

 although it existed, was scarcely appreciable. We shall revert 

 to this fact, Avhich is of importance, presently. 



Foui'tli Experiment. — The following series of experiments 

 were undertaken to enable us to form some idea of the rapidity 

 with which oxygen is absorbed by wort. 



We employed three flasks, A, B, C, of the following capa- 

 cities : — 



A = 234 



B -214 



C = 203 



hich we introduced the following quantities of wort 

 wort, without air) : — 



into w 

 (boiled 



Into A 96 c.c. 



„ B 84 „ 



„ C . . . . . . . . . . 84 ,, 



The necks of the flasks were then drawn out and sealed in a 

 flame, the liquid being at a temperature of 5° C. (41° F.). The 

 flasks were then placed in a hot-water bath and kept at 100' C. 

 (212" F.) for a quarter of an hour. The flask A was repeatedly 

 shaken during cooling, as also was the flask B, this being 

 omitted in the case of the flask C 



The contents of flask A were submitted to analysis as soon as 

 it was quite cooled — that is to say, in about three hours. The 

 analysis of contents of B and C was delayed for about twenty- 

 four hours. We took the precaution of not commencing the 

 analysis before we had shaken the flasks for a few minutes, so 

 that the wort in all of them might be saturated at a flxed 

 temperature, and thus enable us to ascertain the exact quantity 

 of oxygen in solution. 



