FATE OF TVUERCLE BACILLI OUTSIDE THE ANIMAL BODY 301 



6 Continued 



Author 



Date 



Conditions 



Not killed 



Killed 



Kirchner * 

 (continued) 



Cornet 26 



Laschtsch- 

 enko 78 



Neisser 102 



Beninde 18 



1893 



1898 



1898 



1899 



Cornet 86 



Sticker 139 



Jones 84 



1899 



1899 



1900 



Peterson 1 



Heymann 59 



1900 



1901 



ies three rooms were investigated, 

 from which 8 guinea pig's were in- 

 oculated; one died early and one 

 of the other 7 became tuberculous. 



Tuberculous sputum placed on a car- 

 pet and allowed to dry naturally 

 produced tuberculosis in 35 out of 

 36 guinea pigs which were placed 

 in a room at a distance of 134 to 

 300 cm. from tbis carpet 



Tuberculous sputum dried in thick 

 Jayers on paper and cloth was not 

 virulent after 9 months 



Tuberculous sputum rubbed up with 

 dust and aspirated at a velocity 

 of 3 to 5 cm. per second will pro- 

 duce tuberculosis, when inocu- 

 lated into guinea pigs, in a large 

 majority of cases 



Handkerchiefs that were used by con- 

 sumptives from 2 to 12 hours were 

 dried for 24 hours, and then rubbed 

 up in a box. The dust from these 

 was aspirated and injected into two 

 guinea pigs for each test. In 12 

 tests 8 were positive and 4 nega- 

 tive 



Dried sputum in one case lost viru- 

 lence in 3 months; another time 

 remained virulent from 6 to 8 

 months . 



Tested 29 samples of sputum dried from 

 2 to 21 days, by inoculating guinea 

 pigs with dried sputum dust and 

 by causing them to inhale the 

 dried sputum dust. Tuberculosis 

 was produced in 27 out of 29 tests. 



The author inoculated guinea pigs 

 interperitoneally with the mucus 

 from the nostrils of 131 healthy 

 persons. Three of these guinea 

 pigs died of tuberculosis on the 

 8th, 14th and 59th days after in- 

 oculation. No tubercle bacilli 

 could be found by stains of the 

 nasal mucus ' 



One of 8 guinea pigs became tuber- 

 culous after inhaling sputum, 

 dried for 2 months; while sputum 

 dried for 3 months gave one posi- 

 tive in 6 



The aspirated air from sputum, dried 

 6 x days and injected into guinea 

 pigs, produced tuberculosis .... 



.4 percent 



8 positive 



8 mo. 



Positive 

 27 of 29 

 tests 



9 mo. 



4 negative 



3 mo. 



2 mo. 



3 mo. 



6 days 



'It is probable that these three guinea pigs, or at least the first two, died of 

 spontaneous tuberculosis, 



