FATE OF TIT.KRCLK BACILLI OrTsini. TIIF. ANIMAL BODY 313 



isms. Cultures on glycerine egg and the inoculation of a second 

 guinea pig gave negative results. Guinea Pig 1028 was killed 41 

 days after inoculation, and the autopsy showed a small abcess at 

 the point of inoculation and several lesions in the liver. All other 

 organs were apparently healthy. A stained preparation revealed 

 several tubercle bacilli a part of which showed signs of disintegra- 

 tion. Glycerine egg media and a guinea pig were inoculated with 

 pus material from the point of inoculation and a liver abcess. 

 Neither of these revealed living tubercle bacilli. 



A third experiment was made using the same 

 EXPERIMENT III strain of tubercle bacilli. An emulsion was made 



in sterile 0.8 percent salt solution. The emul- 

 sion showed a faintly milky appearance. Three tubes of glycerine 

 egg were seeded with this emulsion of tubercle bacilli and Guinea 

 Pig 1666 was injected interperitoneally with i cc. The remaining 

 portion of the emulsion was divided into two parts : one was 

 heated at 85 C. for ten minutes, the other was heated in the 

 autoclave at 115 C. for ten minutes. With each of these portions 

 three glycerine egg slants were seeded and a guinea pig was in- 

 oculated interperitoneally with i cc. The unheated portion gave 

 excellent growth and produced severe generalized tuberculosis in 

 Guinea Pig 1666. The part heated to 85 C. for ten minutes gave 

 no growth on either of the three glycerine egg tubes after six 

 weeks incubation at 38 C. Guinea Pig 1167, killed 67 days after 

 inoculation, showed local tuberculosis in the right superior inguinal 

 near the point of inoculation and a few small lesions in the liver. 

 All other organs were apparently healthy. A stained preparation 

 from pus! material of these lesions showed tubercle bacilli. Cul- 

 tures on glycerine egg and the inoculation of a second healthy 

 guinea pig from this material gave negative results. The part 

 heated to 115 C. for ten minutes neither gave growth on the 

 glycerine egg media nor produced any effect when i cc. was in- 

 jected interperitoneally into Guinea Pig 1168. 



i. Dead cultures, when not killed at too high a 

 CONCLUSIONS temperature, produce tuberculous lesions in guinea 

 pigs. 



2. Secondary guinea pigs inoculated from tuberculous material 

 from lesions produced by dead cultures always remain healthy. 



3. In determining the length of time tubercle bacilli live when 

 exposed to various conditions outside the animal body, it is nec- 

 essary to inoculate a second healthy guinea pig, especially when 

 only local lesions are produced in the guinea pig inoculated with 

 the original material, in order to be sure the tubercles are not 

 produced by dead tubercle bacilli. 



