316 



BULLETIN No. 161 



[November, 



The glycerine egg was prepared by opening, aseptically, ten 

 good fresh eggs and pouring the whole contents into a sterile flask. 

 To the well-beaten eggs there were added 200 cc. of 5 percent gly- 

 cerine beef broth. This emulsion was placed in sterile test tubes 

 by means of a sterile Pasteur's bulb pipette. It was solidified, in 

 a slanting position, at 73 C. for two hours. After placing the 

 exposed slip on the surface of the media, it was rubbed and scraped 

 by means of a small sterile platinum spatula so as to dislodge some 

 of the dried tubercle bacilli, and a small amount of the glycerine 

 egg or glycerine agar was smeared over this slip until it was 

 thoroly moistened. The cotton stoppers were flamed, dipped into 

 parafrme and pushed into the tubes, which were then stopped with 

 paraffined stoppers. The cultures were incubated at 38 C. for one 

 month and then carefully examined for growth. 



Table 9 indicates the method of recording the re- 

 RESULTS suits for the separate tests. A summary of the 



results of the various tests from the three types 

 of cultures is given in Table 10. It is seen that these germs are 

 killed in a very short time (i to 4 minutes). These results agree 

 with those of Weinzirl (see Table 2). 



9. BACILLUS TUBERCULOSIS, HUMAN TYPE EXPOSED TO SUNSHINE 

 (Between 11 and 12 A. M., Nov. 2, 1911) 



