15. As would be expected, these germs exposed in cow manure 

 retained their virulence longer in the shade than in the sunshine, 

 as shown both by the greater severity <>!" the disease produced in 

 the guinea pigs inoculated with the germs exposed in the shade, 

 than that produced in the guinea pigs inoculated on the same day 

 with the germs exposed in the sunshine, and by the greater length 

 of time that the guinea pigs which were inoculated with the germs 

 exposed in the sunshine remained alive. Pages 325-326 



1 6. Tubercle bacilli in the manure of a naturally infected cow 

 exposed in the same manner as the artificially infected manure 

 were dead within two weeks after exposure. Pages 327-334 



17. Tubercle bacilli in garden soil and in a dead tuberculous 

 guinea pig buried in garden soil were alive on the 21 3th and the 

 7 ist days, respectively, and dead on the 23Oth and 99th days, 

 after first exposed. Pages 334-339 



1 8. Tubercle bacilli live for more than a year in running water. 

 A watering trough harboring these germs may be a dangerous 

 source of infection to cattle. The better disposition of dead tuber- 

 culous animals is to destroy by burning. Tubercle bacilli in drink- 

 ing water is one of the possible sources of infection for man. In- 

 fection is not prevented by dilution, since clumps containing a 

 great number of these organisms may be inclosed in mucoid ma- 

 terial which prevents their separation and destruction. 



Pages 340-359 



19. Tubercle bacilli in market butter placed in cold storage live 

 for more than ten months, which is a longer time than such butter 

 is usually kept in storage. Pages 359~363 



20. General discussion. Pages 364-365 



21. References. Page 366 



281 



