/p/.?] FATE OF TUBERCLE BACILLI OUTSIDE THE ANIMAL P>OI>Y 323 



of the test; the number of clear days during this time and also the 

 number of partly and of wholly cloudy days. There is also given 

 the number of hours of sunshine, the amount of rainfall, the 

 highest maximum daily temperature and the lowest minimum daily 

 temperature occurring between each test; and an average daily 

 maximum, and an average daily minimum temperature during the 

 time of exposure up to the time the test was made. There is 

 also given the average monthly temperature occurring during the 

 time of exposure. 



RESULTS OF THE TESTS 



FROM ARTIFI- Exposed in the Sunshine. The results of testing 

 FECTED IIS tne sam pl es f cow manure infected with a pure 



MANURE culture of tubercle bacilli exposed in the sunshine 



are given in Table 13. As previously mentioned 

 (page 320), the test of the sample on July 29, 1910, the day it was 

 first exposed, produced two severely tuberculous guinea pigs. Since 

 exposure seven tests have been made. On the 7th and i6th days 

 the four guinea pigs inoculated contracted severe generalized tuber- 

 culosis. Only miscroscopic and cultural tests were made from the 

 two guinea pigs inoculated on the 7th day of exposure, since the 

 infection in both guinea pigs was so severe it appeared certain to 

 be from living tubercle bacilli. This was later shown to be true 

 from the culture tests. Microscopic and culture tests from the 

 diseased tissues of both of the tuberculous guinea pigs infected 

 with the sample taken on the i6th day of exposure, as well as an 

 inoculation test from the tissues of one of them, showed charac- 

 teristic tubercle bacilli. The test of the sample made 31 days since 

 first exposed produced in both guinea pigs inoculated only slight 

 tuberculosis of the right superior inguinal lymphatics. Microscopic, 

 cultural, and inoculation tests of the diseased tissues from these 

 guinea pigs showed typical tubercle bacilli. On the 49th day of 

 exposure (September 16, 1910) the test showed that the virulence 

 of the tubercle bacilli had considerably decreased, but was sufficient 

 to produce slight tuberculosis in one of the two guinea pigs in- 

 oculated. The guinea pigs were inoculated subcutaneously with 

 the centrifuge sediment of 40 cc. of an emulsion made by thoroly 

 grinding three grams of the dried sample of infected manure in 

 150 cc. of 0.8 percent salt solution. The pus from the right supe- 

 rior inguinal of the guinea pig that became tuberculous was shown 

 by miscroscopic, cultural, and inoculation tests to contain charac- 

 teristic tubercle bacilli. The other guinea pig, when killed 53 

 days after inoculation, was found to be healthy. Tests made on 



