1912] FATE OF TUBERCLE BACILLI OUTSIDE THE ANIMAL BODY 327 



FROM NATUR- The tests of the samples of manure from the 

 tuberculous cow, both the one exposed in the 

 shade and the one exposed in the sunshine, at no 

 time after exposure produced tuberculous guinea pigs. The re- 

 sults are tabulated in Tables 15 and 16. The sample exposed in 

 the sunshine was tested on the I3th, 34th, 63d, and io5th days 

 after first exposure. All the guinea pigs save one, which died of 

 acute infection in four days, remained healthy until killed and 

 examined 49 and 52 days after inoculation. The sample exposed 

 in the place protected from sunlight was tested on the same days 

 as the one exposed in sunshine, with one exception. No test was 

 made on the I3th day of exposure from this part of the infected 

 manure since there were not a sufficient number of guinea pigs 

 available at that time. This sample was omitted because it was 

 thought that the tubercle bacilli in the part protected from the 

 sun would be the least likely to die. It was indeed unexpected that 

 these bacilli would be dead in either of these two sampled at this 

 time. It was a hot time in August. During the exposure from 

 August 1 6 to 29 there was an average temperature of 72.32 F. 

 and a rainfall of 2.32 inches, having six clear, one wholly cloudy, 

 and seven partly cloudy days. A number of showers occurred, 

 making it an excellent time for the growth of decay organisms 

 found in the manure. 



The killing of the tubercle bacilli in so short a time was no 

 doubt due partly to the antagonism of the decay organisms and 

 partly to the weakened virulence of these germs. Slight virulence 

 was shown by the producing of only localized tuberculosis in one 

 of the two control guinea pigs inoculated with a sample of the 

 fresh manure. More experimental data upon this subject is very 

 desirable. 



