FATE OF TVHERCLE BACILLI OUTSIDE THE ANIMAL BODY 



341 



time some algae and bacteria present in this water, a part of which 

 were still living at the last test made. 



DDc-DAQA-rirkM The emulsions both of the bovine and of the 



I IV IN 1-111 M1- 1 



OF EMULSIONS human cultures of tubercle bacilli were prepared 

 in the same way. About two milligrams of a four 

 weeks' culture grown upon glycerine agar were emulsified in the 

 manner described under "Cultures and Emulsions," page 315. The 

 emulsion of bovine tubercle bacilli was diluted to 350 cc. with 

 the tank water obtained for this purpose; 250 cc. were used to 

 expose in the running water, and 100 cc. to expose in standing 

 water. In a similar way a 250 cc. emulsion of human tubercle 

 bacilli was made. 



EXPOSURE OF 

 SAMPLE 



For exposing the emulsions in running water two 

 6-inch flower pots were obtained. The small 

 opening in the bottom was stopped with a cork 

 and this was sealed over with boiler paint. A test of the two 

 flower pots thus prepared showed that they held water. After 

 immersing them for twenty-four hours to within two inches of 

 the top, no water rose on the inside, tho it became very moist. The 

 two emulsions were now poured into these two flower pots and 



FIG. 1. THE VESSELS AND THE POOL OF RUNNING WATER IN WHICH THE 

 TUBERCLE BACILLI WERE EXPOSED. 



The intake is at A; the outlet at B. The submerged vase C has been placed 

 upon the shelf with other vessels so it can be seen. The flower pot D, 

 which contained the tuberculous guinea pig-, is placed upon a brick to 

 bring it into view. The two unglazed cylinders and one of the flower 

 pots show the effect of freezing. 



