1912] FATE OF TUBERCLE BACILLI OUTSIDE THE ANIMAL BODY 355 



The Diseased Tissues of a Tuberculous Guinea Pig 



In order to test the length of time that tubercle bacilli will 

 live when exposed in running water, Guinea Pig 918 was selected. 

 This guinea pig, inoculated with tubercle bacilli of the human type, 

 was killed 47 days after inoculation and found to be severely 

 tuberculous. The autopsy showed the liver and the spleen to be 

 much enlarged and thickly set with yellow masses of tubercles; 

 the lungs and lymphatics were also extensively infected. Micro- 

 scopical and cultural tests showed the tubercle bacilli to be char- 

 acteristic and active. 



MANNEROF The dead tuberculous guinea pig was placed in a 

 EXPOSURE flower pot having the opening in the bottom 



closed. A half brick was placed upon the guinea 

 pig to keep it it from floating up when placed in the running water 

 of the pool. The flower pot was set on the bottom of the pool, 

 which was twenty inches deep. 



RESULTS ^^ e resll l ts are given in Table 28. All the tests 



OF TESTS made from the decaying and putrefying tissues 



of this guinea pig with one exception produced 



FIG. 4. GENERALIZED TUBERCULOSIS IN GUINEA PIG 1126 INOCULATED WITH TUBERCU- 

 L(>rS SlTTl'M AKTEK BEING EXPOSED IN RUNNING WATER FOR 187 DAYS. 



