8 Objects Seen in Cholera Evacuations. [part i. 



and the large one broke up into air-globules and granular matter (3, 4). Pressure 

 was again applied, and the small one also broke up, as shown at 5. Another case 

 may be quoted as illustrative of this kind of cyst. The evacuation was passed six 

 hours after attack, and two hours before death ; it contained numerous cyst-like bodies, 

 some entire, others more or less broken up, and in many cases seemed to contain 

 partitions (Plate III, Fig. vii). These bodies withstood the action of rectified ether 

 until the fibro-albuminous matter surrounding them had been removed by the 

 application of potash. 



2. In the same evacuation other globular cyst-like bodies were found of a yellowish- 

 green tint, having a more defined outline, and more evenly diffused contents (Plate III, 

 Fig. viii, 1, 4). These were unaffected by the ether, and remained unaffected by liquor 

 potassae for three days. These cysts occurred in nearly all the evacuations examined, 

 but their precise nature was for a considerable time unexplained. They were some- 

 times round, but generally oval, and in some cases formed about a fourth of the entire 

 sediment. This was particularly observed in some dejecta with which I was favoured 

 from the Medical College Hospital, obtained from a native who was admitted with all 

 the symptoms of cholera, but eventually recovered. They were, as in other cases, of 

 a greenish-yellow tinge, with colourless hyaline capsules, for the most part oval (x) — 

 sometimes round, and varying considerably in size, as seen in the figure. One of these 

 cysts was selected for special observation, the one represented at Fig. ix, 1 ; ether being 

 added, the contents cleared up a little, but nothing further; this was followed by 

 strong liquor potassse, which caused it to become dotted and streaky, the yellow tinge, 

 however, remaining (2) ; gradually changing to the appearance depicted at 3, the centre 

 becoming more transparent than the circumference, which still further extended, as at 4. 

 The transparency of the central portion diminished in the course of a few minutes (5), 

 in which condition the object was left under the microscope until the next morning, when 

 it was found to have retained its form, but had acquired a dark colour. Another cyst 

 was selected with a dark-yellow granular centre, and hyaline capsule (Fig. x, 1). Ether 

 was added ; scarcely any change ; merely clearing up the centre a little. It was then 

 rolled over, and the granular contents spread throughout the entire cell (2). Firm 

 pressure was applied, the eye being kept steadil}' at the microscope, when suddenly 

 numerous minute molecules escaped (3), and the capsule became partly emptied of its 

 contents (4). Liquor potassse was added to a portion of the evacuation, and allowed to 

 stand all night. The cysts on examination next morning appeared unaffected. 



To another test tube sulphuric acid was added. The cysts, after remaining several 

 hours in the acid, were not much altered, but presented a globular outline with a 

 hyaline capsule surrounding a greenish-yellow molecular mass (Plate IV, Fig. xi, 1, 2). 

 On rolling them over they became oval, but soon regained the circular form. On the 

 addition of a strong solution of iodine, the contents became dark-brown, and on sub- 

 sequently adding absolute alcohol, fat-like globules made their appearance, which, by 

 manipulation could be made to move within the cell ; the capsule being unaffected 



