lO Objects Seen in Cholera Evacuations. [part i. 



ones, however, are those aheady described, namely, (1) comjpound cysts., consisting of 

 fragments of various tissues and fat surrounded by a semi-organized iibro-albuminous 

 layer, and (2) ova of various kinds, none of which are peculiar to cholera. 



As, however, the ultimate elements of other cysts than these might exist in the 

 dejecta, every known method was resorted to for the purpose of developing them, a 

 few illustrations of which I give in a condensed form. 



Illustration I. : — 



Small portions of the dejecta which contained such numbers of the cysts, alluded 

 to in page 7 and represented at Plate III, fig. v, were placed in three perfectly clean 

 watch-glasses with the following substances : — 



I. — Cholera evacuation 3 drachms, and 2 drops of acetic acid, so as to neutralize it. 



II. — Cholera evacuation 3 drachms, phosphate of ammonia 3 grains, grape-sugar 

 3 grains. 



III. — Distilled water 3 drachms, phosphate of ammonia 3 grains, grape-sugar 

 3 grains. 



To receive these, a small wire stand had been placed in a shallow dish containing 

 a strong solution of permanganate of potash, and the stand and watch-glasses covered 

 in by a bell-glass (carefully cleaned, and subsequently rinsed with alcohol) which stood 

 in the fluid. This was set aside in an average temperature of 82° Fahr. 



On the third day small white specks were seen on the surface of No. I., which had 

 returned to its alkaline condition, one of which was picked out as rapidly as possible 

 from beneath the bell-glass and placed on the stage of the microscope. It consisted 

 of an aggregation of minute molecules held together by a slimy substance, from which 

 filaments of fungi escaped (Plate IV, Fig. xv). Thus matters stood until the fifth day, 

 when from No. II. [a speck] being picked out, presented numerous spores (Plate V, 

 Fig. xvii, 1), many of them germinating very actively (2), and the filaments here and 

 there were swollen out into macroconidia (3, m), some of these dilatations being 

 transparent, others granular ; frequently the filaments were seen to terminate in a 

 bulb (4), and in one case a filament was tipped by a cyst in which the contents 

 were granular and had contracted from the capsules (5). Precisely similar filaments 

 and dilatations were found in No. I. but a distinct cyst (or sporangium) could not be 

 seen. This condition lasted until the seventh day, when the mycelium gradually 

 degenerated, and a crop of aspergillus appeared on all three, of various colours, but 

 principally of the dark varieties. 



Illustration II. : — 



A portion of the fluid contents of the small intestine from a patient who had 

 died within six hours of attack was carefully transferred to a vial, and allowed to settle 

 for an hour. In the meantime a " growing solution " was made, consisting of grape- 

 sugar 3 grains, phosphate of ammonia 10 grains, glycerine 1 drachm, and distilled 



