14 Objects Seen in Cholera Evacuations. ][part i. 



isolating appartus in connection with the aspirator, as already described, the greatest 

 possible care having been taken to prevent foreign matter coming into contact 

 with it before depositing it on the stage in the apparatus. A small portion of the 

 same substance was placed on a glass slide, without any special precautionary 

 measures being taken to prevent access of foreign matter, so as to be able to 

 examine it from day to day for comparison with the preparation in the bell-glass, 

 which it was not intended to disturb. On the second day a few small white spots 

 were observed on both preparations, one of which was picked out with a needle 

 from the non-isolated mass, and placed on the stage of the microscope. It consisted 

 entirely of minute molecules, round and elongated (Plate VIII, Fig. xxi, 1), embedded 

 in a white shining substance (2), in connection with which were circular and oval 

 cells of a greenish tint (3) ; frequently two or more were seen strung together (4) ; 

 clear spaces were seen in them all nearly. 



On the fourth day the mass in the apparatus was completely coated by this 

 white humus, except that some of the earlier observed spots had acquired a yellowish- 

 brown colour. The exposed slide presented a somewhat similar appearance. The cells 

 had become nearly everywhere strung together, and long filaments of oidium lactis 

 (Fig. xxiii), corresponding exactly to the figures given by Thome of the cholera fungus 

 discovered by him, to which rather a long name was given at the time, viz., " Cylindro- 

 tcenium Gholerce Asiaticw." 



This condition lasted till t'he sixth day, when a crop of a white mould was 

 perceptible in the isolated preparation, and a plentiful crop of penicilliuin and 

 aspergillus appeared on the other cultivation (Fig. xxii). The slide having become 

 rather dry, a few drops of distilled water were after this occasionally added. On 

 the eighth day long delicate filaments were seen growing out of the white humus- 

 looking substance in the apparatus, and on the tenth day other filaments were 

 observed, which seemed to be tipped with various coloured heads, apparently of the 

 same kind as on the other slide, those of a bluish and yellowish-brown tint prevailing ; 

 but by the eighteenth day the long delicate filaments had grown over them, the whole 

 surface of the preparation presenting a woolly appearance. After this no further 

 change could be seen to take place in either cultivation, and on the twenty-first day 

 of the experiment the bell-glass was opened, and the glass plate placed on the stage 

 of the microscope. Precisely the same species of aspergillus and penicillium were 

 found as existed in the non-isolated cultivations, with the addition that great numbers 

 of the filaments forming the white flocculent tuft bore at their terminations cysts or 

 sporangia filled with distinct spores (Plate IX, Fig. xxiv, 1-4), which, I think, correspond 

 exactly to the cysts figured by Professor Hallier of the immature cholera-cysts, whose 

 drawing has already been given, and may be compared with this.* 



Aspergillus tufts were present in great numbers : nearly all of them had fallen 



* I have obtained excellent examples of this fungus (^Mricor) on the intestinal mucous membrane of the 

 pig also, whilst subjecting strips of the intestine to continuous observation. 



