PART I.] Effects of Re-agents on the Globules and on '' Spore sT 17 



in a few instances, a slightly granular appearance was presented (2), and the ring was 

 often seen particularly granular (3, 4, 5), as if all the contained granules had adhered 

 to it. The globule in the centre of the figure, with the contents separated from its 

 enclosing pellicle (6), was watched for a long time, but no alteration in its appearance 

 occmred. Other similar bodies were watched continuously for three hours with the 

 same result, save that they gradually became excessively transparent, visible only by 

 careful adjustment of the mirror. In the course of four or five hours the entire field 

 presented the appearance delineated in the figure last alluded to. At Plate XI, 

 Fig. xxxiv, a regular colony is seen of these globules surrounding a crystal. They 

 also disappeared in the course of a few hours. 



Rectified ether caused the pellicle to present a minute granular appearance, and 

 those which had the contents puckered became symmetrical. Boiling in ether seemed 

 to thicken the pellicle. A portion of this was set aside until the next day, and was 

 found to have retained its condition, whereas the globules in the evacuation set aside 

 in the vial had disappeared. 



Absolute alcohol subsequently added to the boiled portion seemed rather to diminish 

 their number. In some cases one globule was observed to " melt " into the other, so as 

 to form one globule ; otherwise no change was observable. 



Solution of chloride of zinc and iodine, — some became shrunken and irregular, 

 others continued spherical, but with a finely granular pellicle. 



Solution of iodine only caused several of them to become very transparent — 

 scarcely visible, were it not for the slight tint communicated to them. 



Liquor potassoe causes them to lose their yellow colour ; they become perfectly 

 transparent, except that a few molecules which existed within are brought to view. 

 A few of the globules withstand the re-agent for some time. 



Acetic acid seemed to coagulate the pellicle, as it became finely granular: very 

 much the same appearance as followed the addition of alcohol. 



Dilute sulphuric acid caused the contents to contract, but the colour was 

 retained, or it became slightly brown. 



Dilute nitric and hydrochloric acids acted in the same way. 



I have made many attempts artificially to produce globules of this kind, the 

 nearest approach being a mixture of melted butter, albumen, and gum-water well 

 shaken together, and at the time of examination adding a little thick syrup so as to 

 cause the puckering to take place between the pellicle and the contained fat. The 

 action of re-agents, however, on this pellicle was slightly different to the foregoing. 



Spores immersed in fluids of varying density become greatly altered in their 

 appearance; frequently the outer layer becomes so attenuated, and perhaps stained, 

 that it is a matter of great difficulty to state positively that the cell pellicle surrounding 

 the protoplasm of a spore differs from the clearly defined outline of a globule of oil, 

 in spite of a knowledge of the action of re-agents, and of the varying powers of 

 refraction which liquids manifest. Hence it is not impossible, nor inexcusable, 



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