34 Objects Seen in Cholera Evaaiations. [part 1. 



On the third day the globules were seen to have increased considerably in number, 

 and on the eighth day germination was rapidly taking place (Plate XVIII, Fig. Ixxiii). 



A little gum-water being added caused the central part to become clear and 

 watery, and the protoplasm to shrink in the mycelium (Fig. Ixxiv). Germination 

 continued for a few days longer, but no more advance stage could be attained. 



{(T) A test tube containing the water used in these observations was also set 

 aside, but nothing developed in it. 



Illustration II. : — 



About a drachm of ordinary fgeces was dissolved in an ounce of distilled water 

 and filtered, a portion of which was placed in a watch-glass and boiled thoroughly ; 

 a drop of this was afterwards placed in an ordinary animalculse growing slide, both 

 being set aside under a bell-glass. 



On the second day monads and vibriones were present in great numbers in both 

 preparations, but on the third day they had greatly diminished in number in the 

 watch-glass, in which, however, during the night several young animalculse belong- 

 ing to the Kolpoda family (as figured at Plate XIX, Ixxx) had made their appearance. 



In the live-box, however, vibriones only were present as before, some of them being 

 very long, but no Kolpodse. On the fourth day great numbers of amoeboid bodies, varying 

 considerably in size (Plate XIX, Fig. Ixxvi, 1), multiplying very rapidly, sometimes by 

 leaving small fragments of their substance behind (2) ; the portion escaping invariably 

 from a part near the contractile vesicle, which vesicle remained bright for fifteen 

 seconds, became puffed out suddenly, as though it had been a taper, and remained 

 extinguished for the same period, then gradually shone again. The detached portion 

 (3) seemed not to be merely disgorged food, for it crept about the field like its 

 parent ; it also divided into two, pretty symmetrical, halves. For some time after the 

 commencement of division, the " nucleus " is only seen in one half (4) after considerable 

 tugging, then coming together, then separating again, each time getting a little more 

 detached, until in the course of about two minutes the separation is complete. Fre- 

 quently a mass of granules is seen to intervene, probably indigestible particles, which 

 may adhere to either half (5), but is soon cast off, and gradually a contractile vacuole 

 is seen to appear in the second half, which creeping along the field draws particles into 

 its substance, and acts in every way like its parent (6). 



On the fifth day the fluid in the slide having somewhat evaporated, a little distilled 

 water was added, when suddenly the hitherto more or less oval amoebae (Fig. Ixxvii, 1) 

 commenced protruding and retracting exceedingly long processes (2), which action lasted 

 three-quarters of an hour. They then became circular and still, except that the vacuole 

 contracted (3). In another half hour some of them commenced to creep along the field, 

 disgorge themselves, leaving a string of granules to mark their path (Fig. Ixxviii, 1) ; 

 others were observed in the course of another half hour to become circular, with a clear 

 halo-like ring surrounding them (2), their contents being in very active motion, reminding 



