PART I.] Developments Seen in a Fresh Cholera Dejection. 39 



which, and above and below, myriads of monads and short vibriones played. Some 

 slight distance from the margin an opaque line, consisting of unusually active monads, 

 was seen separating the creamy ring into an inner and outer portion, but no 

 distinction eould be observed between the appearance of the molecules of one side 

 from that of the other, except that in the outer the heaps were more plentiful. 



The monads, etc., in the central clear space had become perfectly still, and no 

 heaps had formed amongst them ; towards evening the line seemed to have spread 

 on either side, as the whole creamy ring became as thickly studded with molecules — 

 consequently opaque — as the narrow line was in the morning. The circular cells 

 seem to have disappeared altogether. 



On the sixth day not a single molecule quivered. The creaminess of the margin 

 had slightly diminished, but the " heaps " were still present, rather more slimy-looking, 

 not so regularly circular, but frequently elongated and straggling. The diameter of 

 some of the molecules in contact with the mass had increased. 



A mycelial filament was now seen to insinuate itself from without into the 

 preparation, having crept up the " chimney " of the growing slide, and the further 

 development in or about the heaps came to a standstill, although watched for a 

 month. The mycelium spread in every direction, and gave rise to yeast cells. 



(6) A similar slide containing a drop of this stool, to which a little of the 

 growing solution of grape-sugar and phosphates was added, went through the same 

 stages as the foregoing, and developed into penicillium, as in Plate VI, Fig. xix, 2. 

 (c) A portion of the same preparation without a covering glass was preserved 

 in a moist chamber. On the third day a white speck was seen in the surface 

 consisting of innumerable " yeast " cells (Plate XX, Fig. Ixxxvi), with some filaments 

 branching in all directions. On the fourth day tufts of penicillium had developed 

 — two varieties (Plate XXI, Fig. Ixxxviii) — P. Glaucum (1), and P. Viride (2). 

 This continued until the ninth day, when a few of the filaments springing up in 

 the midst of the penicillium were tipped with a dewdrop-like dilatation exces- 

 sively delicate — a mere distended pellicle. In some cases they seemed to be derived 

 from the same filament as others bearing the ordinary branching spores of penicillium, 

 but of this I could not be positive. This kind of fructification increased rapidly, 

 and on the fourteenth day spores had undoubtedly developed within the pellicle 

 (Plate XX, Fig. Ixxxvii), just as had been observed in a previous cultivation (page 

 12), precisely similar revolving movements being also manifested. The reaction of 

 the liquid portion in the cell was slightly acid, and became very much more so in 

 the course of a month. No further change took place, except that the capsules 

 became rather thicker, but never so resistant as to withstand the action of a drop 

 of water, spores being instantaneously set free by it. 



In not a few (cases a chain of spores, or sometimes delicate filaments, seemed 

 to escape from these cysts, as if the spores within had germinated ; which indeed 

 must have been the case, unless they had fallen from a tuft of penicillium and 



