PART I.] Injection of Choleraic Fluid into Veins of Animals. 85 



old rupture of the organ. The larger veins contained fluid blood, and there was no 

 indication of the occurrence of embolism in any part of it. The kidneys were ex- 

 tremely fatty, and the left one contained one or two embolic masses of considerable 

 size, projecting on the surface and extending deeply into its substance. The bladder 

 was full of urine. The pleurae, lungs and heart were perfectly normal in aspect. The 

 blood showed no traces of bacteria in it when carefully examined a quarter of an hour 

 after its removal from the heart. 



(6) The choleraic Tnaterial used being one day old. 



Experiment II. — A dog which had been previously the subject of injections of 

 choleraic media, both femorals and one basilic vein having been previously tied 

 (vide Experiments VI, XXXVI and XL), but which was nevertheless in very fair 

 condition, was put under chloroform at 7-30 a.m. The remaining basilic vein was 

 now opened, and four drachms of a choleraic evacuation passed by a patient in 

 hospital 24 hours previously, and which had remained in a bottle during the 

 interval, were injected into it. 



The animal rapidly recovered from the effects of the chloroform, and, saving 

 that it limped slightly in walking, appeared to be in no way affected by the 

 operation. It continued apparently in health throughout the day, walking about 

 and feeding, and on the following morning also it showed no symptoms of illness. 

 It was therefore killed under chloroform as in the previous experiment, and an 

 immediate 'post-mortem, examination performed. 



There was a localised sac of pus in the sheath of the vessel last injected. 

 The pus appeared to be perfectly normal, and there was little inflammation around 

 the sac. The wounds caused by the previous operations were clean and healthy, 

 that in connection with the femoral vein of the opposite side being almost entirely 

 healed up. The abdominal and thoracic organs were carefully examined, but save 

 for the existence of one or two minute, congested, possibly embolic patches in the 

 liver, they appeared healthy and normal. 



Experiment III. — A healthy young pariah dog was put under chloroform at 7 a.m., 

 and four drachms of choleraic evacuation, which had stood for 24 hours in a 

 bottle, were injected into the right femoral vein. The operation was performed on 

 the same morning and with the same material as Experiment II. 



The animal once or twice ceased to respire during the operation, but, by resorting 

 to artificial respiration, sensibility was restored, and it eventually recovered from the 

 influence of the chloroform. 



Several days of illness supervened, during which the animal appeared hardly 

 able to walk, became much emaciated, and was affected with slight convulsive* 

 twitchings. These symptoms gradually passed off, and at the close of a week aftei' 



