86 Researches Regarding Cholera : The Blood. [part i. 



the performance of the operation, it appeared to have entirely recovered. It 

 was accordingly killed on the morning of the eighth day, and an immediate 

 'post-Wiortem examination made. 



On opening the abdomen the peritoneal cavity was found to contain a consider- 

 able quantity of reddish serous fluid. The intestines were congested externally, and 

 contained here and there patches of reddish mucus. The remaining abdominal and 

 thoracic organs appeared to be perfectly healthy. 



(c) — The choleraic material used being two days old. 



Experiment IV.^ — A large, healthy young pariah dog was put under chloroform, 

 and about half an ounce of choleraic material was injected into the right femoral 

 vein. The material employed consisted of the supernatant fluid of an evacuation, 

 which had stood for 40 hours, and which had, when quite recent, been chiefly 

 characterised by the profusion of large, active amoeboid bodies present in it. 



The dog rapidly recovered from the influence of the chloroform, and appeared 

 to be quite unaffected by the operation. On the following morning, 24 hours after 

 the injection, there were no symptoms of illness, and during the subsequent three 

 days the animal appeared to be in perfect health, so that, on the morning of the 4th 

 day from the first operation, he was made the subject of another experiment {vide 

 Exp. XXII). 



Experiment V. — A very young and healthy pariah pup was put under chloroform, 

 and two or three drachms of the supernatant fluid from a choleraic evacuation 

 which had stood in the laboratory for 48 hours were injected into the right 

 femoral vein. 



The animal recovered rapidly and perfectly from the influence of the chloroform. 

 It showed no symptoms of illness, and on the following day appeared to be quite 

 well. As it continued in perfect health, it was subjected to a fresh operation 48 

 hours after the performance of the former one (vide Exp. XXXV). 



Experiment VI. — A healthy pariah dog, into the right femoral vein of which 

 choleraic material had been injected three days previously without result, was put 

 under chloroform, and four drachms of a dejection, which had been passed 48 hours 

 before by a patient who had been ill for 24 hours, were injected into the femoral vein 

 of the opposite side. 



The dog was never fully under the influence of the chloroform, and very soon 

 appeared as though nothing had happened to it. On the following day it seemed to 

 be quite well and remained so until after the lapse of three days, when it was again 

 operated on {vide Experiment II). 



