PART I. "I Injection of Choleraic Fluid into Veins of Animals. Z'] 



(d) —The choleraic material used being three days old. 



Experiment VII.— A strong healthy pariah dog was put under the influence of 

 chloroform, and four drachms of choleraic fluid were injected into the left femoral 

 vein. The fluid was derived from an evacuation which had been kept for 72 hours.* 

 It was watery, and at the time of the operation had but a very slightly offensive 

 odour and a faint alkaline re-action. There was hardly any sediment present, and 

 the material injected consisted entirely of liquid crowded with very large and active, 

 stiff-looking bacteria, together with myriads of active flagellated 'monads. 



The animal appeared to be very little affected by the operation, showed no 

 symptoms of disease throughout the day, and by the following morning seemed to be 

 quite well. Chloroform was accordingly again administered, and continued until death 

 occurred 24 hours subsequent to the performance of the injection. 



A post-mortem examination was performed at once, the abdomen being opened 

 before respiration and circulation had finally ceased. There were no signs of 

 peritonitis present. The mucous surface of the intestines appeared to be quite 

 healthy. Scrapings from it showed merely normal epithelial cells and villi with 

 the usual sprinkling of minute bacteria. The mesenteric glands were congested and 

 contained a good deal of fluid in their interior. A preparation of this fluid was 

 mounted in a wax cell and examined one hour and a half afterwards. It was found to 

 consist of a clear liquid, containing no recognisable bacteria or vibriones, but full of 

 red blood-corpuscles and active amoeboid bioplasts. It was again carefully examined 

 24 hours later, but the only change observable in it was that the bioplasts had all 

 become motionless and circular. The rest of the abdomen and thoracic organs were 

 apparently healthy. 



Two preparations of blood from the heart were mounted as usual in wax cells. 

 These were examined about an hour afterwards, and again after the lapse of 24 hours. 

 On neither occasion did they show anything abnormal, the serum was perfectly clear 

 and free from all trace of bacteria or vibriones, and an abundance of leucocytes crawled 

 out of the clot and subsequently underwent their usual changes. 



Experiment VIII. — Immediately after the injection of the previous experiment 

 had been completed, another very large healthy pariah dog was put under the 

 influence of chloroform, and six drachms of the same fluid were injected into the right 

 femoral vein. 



The animal rapidly recovered from the influence of the chloroform, and did not 

 at first appear to be much affected by the operation, being able to walk to the 

 kennel with apparent ease. It rapidly, however, became much depressed, and died 

 within three hours. A post-mortem examination was performed five hours after death. 



* The case from which it was derived was la very slight one, rather of choleraic diarrhoea than of 

 cholera, and made a rapid recovery. 



