PART I.] Injection of Choleraic Fluid into Veins of Animals. 89 



exam in ed an hour afterwards. At that time no bacteria could be detected in it, but 

 crystals had begun to appear, and the serum was stained with the colouring matter 

 of the red-coqjuscles, whilst sixteen hours afterwards the whole of the preparation was 

 converted into a mass of large blood-crystals. 



Experiment IX. — On the completion of the preceding operation, Experiment VIII, 

 another very large pariah dog was put under the influence of chloroform, and one 

 ounce of the fluid employed in it was injected into the right femoral vein. 



The animal at first appeared to be very little affected, and, like the dog of the 

 preceding experiment, walked off to the kennel with ease and without manifesting 

 any symptoms of pain or inconvenience. It died, however, within four hours, and a 

 post-mortem examination was performed four hours after death. 



Post-mortem, rigidity was strongly marked. On opening the abdomen the small 

 intestines were found to present precisely the same appearances, both externally and 

 internally, as in the preceding case. There was the same separation of the epithelium, 

 and the resulting formation of a soft, pink, creamy substance, and in this case also 

 the portion of the gut immediately above the ileo-coecal valve was apparently 

 unaffected. 



The only feature in which the intestines in this instance differed from the others 

 consisted in there being a certain amount of watery fluid present in them. Towards 

 the lower end of the ileum, just above the unaffected portion of the mucous 

 membrane, the separation of the epithelium was not so far advanced as higher up, 

 and appeared to be in a state of transition towards such a condition. 



The mesenteric glands were highly congested internally, of a soft consistence, 

 and contained an abundance of pink fluid. A preparation of this fluid was mounted 

 as usual in a wax-cell. When examined an hour and a half subsequently, it was 

 found to contain an abundance of minute bacteria, a sprinkling of the long vibriones 

 occurring in the glands and intestines of the previous case, together with normal 

 gland-cells and numerous red blood-corpuscles. Sixteen hours later, it contained an 

 abundance of minute active bacteria, together with molecular matter, fatty crystals 

 and cells, but showed none of the elongated vibriones previously present in it. 



The large intestine and appendix vermiformis were normal in aspect, and the rest 

 of the abdominal viscera appeared healthy. The bladder contained a little urine. 



On opening the thorax there were found to be numerous large cancerous nodules 

 throughout the substance of both lungs, which were collapsed, but contained a little 

 air. There was slight inflammation of the parietal pericardium, and the visceral 

 layer also was somewhat injected. 



A preparation of blood was, as usual, procured from the right ventricle and 

 mounted in a wax-cell. When examined about two hours afterwards, the serum was 

 found to be deeply stained with the colouring matter of the red corpuscles, and to 

 contain numerous minute active particles, but no distinct bacteria could be detected 



