90 Researches Regarding Cholera : The Blood. [part i. 



in it. Sixteen hours afterwards it was crowded with large blood-crystals and contained 

 an abundance of minute active bacteria. 



Experiment X. — A small healthy pariah dog having been put under the influence 

 of chloroform, about three drachms of the same fluid employed in the three preceding 

 experiments, but which had now been kept for nearly eight hours longer, were injected 

 into the right femoral vein. The injection was performed at 4 p.m., and the animal 

 died during the night. A 'post-morte'm examination was performed at 7 a.m. of the 

 following day. 



Post-mortem rigidity was well marked. There was a little reddish serum in the 

 peritoneal cavity, but the intestines and mesentery were not congested. On laying 

 open the small intestine, the interior was found to present the usual appearances, 

 the portion immediately above the ileo-coecal valve being unaffected, and the rest of 

 it covered with a soft pink coating of detached epithelium. 



Preparations of this pink material as well as of matter scraped from the subjacent 

 denuded mucous coat were examined. The former were found to consist of cylindrical 

 epithelial cells, and to contain numerous long serpentine vibriones similar to those 

 found in the intestines and glands of the dogs of the previous experiments. The 

 latter preparations consisted of imperfectly developed epithelial cells, with an even 

 greater abundance of the elongated vibriones (Fig. 1), which ultimately resolved 

 themselves into a network of leptothrix-filaments (Fig. 2). 



The mesenteric glands contained pinkish fluid, a preparation of which was 

 mounted in a wax-cell. When examined an hour afterwards, the gland-cells were 

 found to be considerably disintegrated, whilst the fluid contained great numbers of 

 the elongated serpentine vibriones, described in the previous preparations, in a state 

 of full activity. The majority of them appeared to be uniseptate with a kind of 

 hinge joint in the middle. 



The rest of the abdominal organs were healthy. 



There was a little reddish serum in the pericardium ; the right side of the heart 

 was full of fluid blood, and the left contained a little also. 



A preparation of blood was as usual mounted in a wax-cell, which was examined 

 an hour and a half afterwards, and found to contain numerous minute molecules in 

 active motion, but no distinct bacteria. Twenty-four hours afterwards it was crowded 

 with large needle-shaped blood-crystals, and the serum had almost dried up. 



(d) — The choleraic material used being four days old. 



Experiment XI. — A small healthy pariah dog was put under the influence of 

 chloroform, and four drachms of the fluid employed in Experiments VII, VIII, IX 

 and X, but which had now been kept for 96 hours, was injected into the right 

 femoral vein. The animal seemed to be very little afl"ected by the operation, and 



