PART I.] Injection of Choleraic Fluid into Veins of Animals. 91 



ran off to the kennel very cheerfully a few minutes after it was completed. It 

 remained in apparent health, and was killed under chloroform about 9 hours 

 subsequently. 



During the administration of the chloroform, tarry liquid escaped from the 

 rectum, and the large intestine was subsequently found to be full of similar 

 material. The small intestine for nearly two feet above the ileo-coecal valve appeared 

 healthy, but above the mucous membrane was coated with a sanguineous layer, 

 the epithelium however was not detached. The mesenteric glands were very much 

 congested and full of reddish fluid. Some of them were reserved in a moist chamber 

 for 14 hours. On sections being made at the close of that period, the gland 

 was found to contain fluid in its interior swarming with active bacteria, and con- 

 taining a sprinkling of long, active, serpentine vibriones similar to those found in 

 the preceding experiments with the same fluid. A preparation of the fluid from 

 the glands was also mounted in a wax-cell at the 'post-mfiortem examination. On 

 examination a quarter of an hour afterwards, it was found to be full of red blood- 

 corpuscles and crowded with minute motionless molecules. Twenty-four hours later 

 many of these particles were in active motion, but there were no elongated vibriones 

 present. The rest of the abdominal and thoracic viscera were healthy, and there 

 were no traces of pericarditis. 



Two preparations of blood, one from the vena portae, the other from the right 

 ventricle, were mounted in wax-cells as usual. They were examined a quarter of 

 an hour afterwards ; the latter contained numerous motionless molecules, the former 

 a few small blood-crystals, but in neither were there any distinct bacteria. They 

 were again examined after an interval of fourteen hours. At this time the pre- 

 paration of portal blood contained numerous moving molecules, and an increased 

 number of crystals, whilst the other preparation was apparently quite unchanged. 



Experiment XII.^ — A young and healthy dog was put under chloroform, and 

 four drachms of choleraic dejection, which had been passed ninety-six hours previously, 

 injected into the right basilic vein. 



The animal came rapidly out of the influence of the chloroform, but its respira- 

 tion was disturbed, violent and gasping. Towards the evening of the same day it 

 began to pass reddish, mucous evacuations, and it continued to do so until 5 A.M. 

 of the following morning, when it died, 22 hours after the operation. 



A post-mortem examination was performed at 7 a.m.- On opening the abdomen, 

 the cavity was found to be free from fluid and the peritoneum was smooth and 

 shining, appearing in every respect to be perfectly healthy. The large intestine 

 was throughout coated with a layer of thick, dark red mucus which ceased 

 sharply close to the ileo-coecal valve. The small intestines showed small patches of 

 red mucus on the interior of the jejunum and ileum, whilst the duodenum appeared 

 to be perfectly healthy. 



