PART I.] Injection of Choleraic Fluid into Veins of Animals. 93 



appearing to indicate that they had been due to an abnormal absorption of fluid 

 at these points. On microscopic examination, the material composing these light 

 coloured spots was found to consist of molecular matter and liver-cells in various 

 stages of disintegration. This spleen was normal in appearance. The kidneys also 

 appeared healthy. 



On opening the chest the pleurse were found to be healthy. The trachea and 

 bronchi were empty and normal. The lungs were collapsed, containing very little 

 air. They appeared to be injected on the surface, contained a little black blood, 

 and presented a somewhat pneumonic aspect. The smaller bronchial tubes con- 

 tained a yellowish frothy fluid. The pericardium was healthy. The right auricle 

 was distended with coagulum, part of which was black, part gelatinous and yellowish. 

 The right ventricle was in a similar condition. The pulmonary artery was full of 

 a similar coagulum. The left auricle contained a little dark clot and the left 

 ventricle was strongly contracted and empty. The pulmonary veins were full but 

 not distended with blood. 



Experiment XIV. — A small dog into both of whose femoral veins aqueous 

 solutions of choleraic material had been previously injected without the slightest 

 result {vide Experiments XXXIV and XXXVIII) was put under chloroform, and 

 two drachms of the supernatant fluid of a dejection which had been kept for 96 

 hours were injected into the right basilic vein. 



The material injected contained innumerable monads, bacteria and vibriones, 

 together with a few amoeboid bodies about the size of white corpuscles. The opera- 

 tion was performed at 7.30 a.m., and the dog quickly recovered from the influence 

 of the chloroform, was able to support himself at once, and very shortly appeared 

 as though nothing had happened to it. The animal continued in perfect health, 

 eating and drinking freely, and having certainly improved in condition during the 

 period in which he had been subjected to operations involving the ligature of both 

 femoral and one of the basilic veins. 



Three days subsequent to the last operation, a specimen of blood was taken 

 for microscopic observation, and the dog was then let loose to return to his native 

 wilds, and no doubt to regret the regular diet and attention which he received 

 during his period of service to science. The blood was carefully examined but 

 without yielding the slightest evidence of the presence of monads, bacteria or 

 vibriones. 



Experiment XV. — A large healthy young dog was put under chloroform, and about 

 seven drachms of the supernatant fluid of an evacuation which had been kept for 96 hours 

 injected into the right femoral vein. Five hours subsequent to the operation the dog 

 died somewhat suddenly, having passed about a pint of liquid evacuation just before 

 death. 



A 'post-'mortem examination was performed four hours after death. The body was 



