PART I.] Injection of Fluid Choleraic Evacuation into Femoral Vein. 97 



serum. Twenty-four hours subsequently the preparations were crowded with active 

 bacteria. 



Experiment XVIII. — This experiment was performed at the same time and with 

 the same material as the preceding one. A large healthy dog having been put under 

 the influence of chloroform, four drachms of the fluid were injected into the right 

 brachial vein. 



In consequence of a failure in the first attempt at injection and of the subsequent 

 slipping of a ligature, the animal was both kept for some time under the chloroform, 

 and moreover lost a considerable quantity of blood. The operation was at length, 

 however, successfully performed. The dog seemed to be much depressed and whined 

 as though in pain for some time. 



During the night, however, he improved greatly and drank some water ; and on 

 the following morning he ate and drank greedily, and seemed to suffer more from 

 stififness of the wounded limb than from any constitutional symptoms. The wound 

 looked clean and healthy, and the animal was bright and lively in appearance. He 

 continued free from any constitutional symptoms, and five days after the operation 

 was set at liberty in full health and excellent spirits. 



Experiment XIX. — A pariah dog of average size put under the influence of 

 chloroform at 7 a.m., and four drachms of the supernatant fluid of a choleraic evacuation 

 which had been kept for 96 hours were injected into the right femoral vein. The 

 dejection from which the material employed was derived was passed by a patient in 

 the Greneral Hospital whilst in profound collapse, and consisted almost entirely of 

 watery fluid, only a very little flocculent sediment being present. These flocculi, 

 when quite recent, showed mere flakes of minutely granular matter with a few hyaline 

 cells and myriads of active infusoria. The material injected was entirely free from 

 particles of sediment and consisted of an opalescent fluid swarming with active bacteria 

 and containing a few molecular flakes and active ciliated infusoria. The operation 

 was performed rapidly and with perfect success, and the dog quickly recovered from 

 the influence of the chloroform. It passed a healthy evacuation soon after the close 

 of the operation and showed no symptoms of pain, but extreme depression persisted, and 

 it died about three hours after the operation, having neither vomited nor been purged 

 during the interval. 



A 'post-mortem, examination was performed within two hours after death with the 

 following results. On opening the abdomen the peritoneal cavity was found to contain 

 no fluid, the membranes showed no evidences of inflammatory action, and the exterior 

 surface of the intestines was extremely pale. They were, in fact, of a whitish colour 

 and were closely packed together. The stomach contained undigested food, and the 

 mucous membrane was pinkish and evenly congested over the whole surface. The 

 mucous membrane of the small intestines was congested from the pylorus to the ileo- 

 coecal valve ; it was deep pink and moist ; and the villi presented the brush-like aspect 



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