'98 Researches Regarding Cholera : The Blood. [part i. 



previously described in other experiments. The surface was coated with a thick layer 

 of whitish, semi-fluid, flocculent matter, which in many cases completely choked the 

 lumen of the gut on a cross section being made, and which on microscopic examination 

 was found to be entirely composed of cylindrical epithelium, mingled with which were 

 a few bacteria. The epithelial cells were in singularly perfect condition, and were either, 

 scattered or in sheets and masses. The large intestine was healthy in appearance. 

 It contained normal foecal matter, and the mucous membrane showed no signs of 

 congestion or other morbid change. The mesenteric glands were of a deep pink hue 

 internally, and contained abundance of fluid of a similar colour. This fluid on micro- 

 scopic examination was found to be crowded with the cells normally present in such 

 glands, and to contain in addition a large number of active bacteria. The latter 

 subsequently multiplied to a great extent in a preparation which was mounted in a 

 wax-cell. 



The liver was very soft in texture, but showed no evidences of the occurrence of 

 embolism. The spleen and kidneys were healthy, and the bladder was full of urine, 

 containing numerous cylindrical epithelial scales and active bacteria. 



On opening the thorax, the pleural cavities were found to contain no fluid, and 

 the membranes were smooth and healthy. The lungs were pale and collapsed. There 

 was no pericarditis. The right cavities of the heart contained dark fluid blood, and 

 the left auricle and ventricle were empty. 



Two preparations of blood were mounted in wax-cells. When examined a few 

 minutes afterwards, one of the specimens was found to contain a sprinkling of minute, 

 active bacteria, whilst in the other only one or two could be detected. Twenty-four 

 hours afterwards, the former specimen was crowded with large active bacteria, which 

 were often arranged in long series and in ramifying flakes. The other preparation 

 also showed a considerable increase in the numbers of bacteria present, although by 

 no means so great as had previously been observed in other similar cases. 



Experiment XX. — A large healthy pariah dog was put under the influence of 

 chloroform at 12 noon of the same day in which the previous operation was performed, 

 and four drachms of the same fluid employed in it were injected into the right 

 femoral vein. The operation was rapidly and successfully accomplished ; there was no 

 haemorrhage, and the animal quickly recovered from the influence of the chloroform, 

 and attempted to make its escape. 



Shortly afterwards it became dull and depressed, and continued in that condition 

 throughout the rest of the day. At 7 a.m. of the following day it was still somewhat 

 dull looking, but was sitting up and drinking water freely, and at 2 p.m. it appeared 

 to have almost entirely recovered from the effects of the operation, and was made 

 the subject of another experiment (No. LVI) from which it also recovered. 



Experiment XXI. — A pariah dog of average size was put under the influence of 



