PART I.J Experiments with Choleraic Matter, Six and Eight Days Old. 99 



chloroform immediately after the completion of the previous experiment, and four 

 drachms of the same fluid employed in it were injected into the right femoral vein. 

 The operation was rapidly and in every way successfully performed. 



The respiration ceased whilst the wound was being stitched up, but was readily 

 re-established, and the animal quickly recovered from the influence of the chloroform. 

 It passed a normal evacuation soon after the operation was completed, and remained in a 

 somewhat depressed condition throughout the rest of the day. On the following morning, 

 however, it was much livelier, and 24 hours after the operation it appeared to be quite 

 well, and was very desirous of effecting its escape, which it succeeded in doing not 

 long afterwards. 



(e) — The choleraic matter used being six days old. 



Experiment XXII. — The dog which had previously been employed in Experiment 

 IV was put under chloroform, and half an ounce of the supernatant fluid of the 

 same dejection previously made use of in Experiment XV was injected into the 

 left femoral vein. The dog appeared to be very little affected by the operation. Four 

 days after he was in excellent health ; but the wound in the thigh was still open. 



Several preparations of blood were made by opening a cutaneous vein, and either 

 treated with osmic acid and acetate of potash, or without the use of any re-agent; 

 but in neither series could any traces of bacteria be detected. 



(/) — Choleraic material used being eight days old. 



Experiment XXIII. — A strong young pariah dog was put under chloroform, and 

 half an ounce of the fluid of the same evacuation employed in the preceding experiment 

 injected into the right femoral vein. Owing to the slipping of a ligature, the dog 

 lost about a couple of ounces of blood during the operation. The haemorrhage was, 

 however, controlled, and the dog recovered from the influence of the chloroform, but 

 died about three hours subsequently. 



A post-mortem examination was made within six hours after death. The abdomen 

 was considerably distended. There were two or three ounces of reddish fluid in the 

 peritoneal cavity, and the intestines were pinkish externally. They were distended 

 with air, and coated with a slimy, pale pinkish material, but contained very little 

 fluid. The liver was pale, and there were already gaseous bubbles beneath the 

 peritoneal coat. 



There was no fluid either in the pleural or pericardial sacs. The lungs were 

 collapsed. The right cavities of the heart were distended with black blood ; those of 

 the left side were empty. 



