lOO Researches Regarding Cholera: The Blood. [part i. 



((/) — Choleraic material used being ten days old. 

 Experiment XXIV. — A healthy, but very young pariah pup was put under 

 chloroform, and about three drachms of the fluid of the same evacuation employed in 

 the previous experiments (but which had now been kept for eleven days) were injected 

 into the right femoral vein. The dog rapidly recovered from the chloroform ; but 

 there was some disturbance of the respiration, more eepecially immediately after the 

 injection. The material injected was crowded with monads and bacteria, and contained 

 numerous circular cells nearly the size of blood corpuscles and with granular 

 contents. 



The dog died 2^ hours after the operation, and a post-mmiem examination was 

 performed six hours afterwards. Rigm^ mortis absent. The abdomen was distended, 

 and the peritoneal cavity contained some reddish fluid. The intestines were distended 

 with air, but contained no liquid. The mucous membrane was, in one or two spots, 

 coated with pinkish mucus. The liver and spleen were healthy in aspect. 



The lungs were collapsed, airless and bloodless. Both sides of the heart were 

 distended. Specimens of blood, both from the heart and from the vena cava inferior., 

 showed no distinct traces of either monads or bacteria, although carefully examined 

 for them. 



(K) — Material used being twelve days old. 



Experiment XXV. — A small pup was put under chloroform, and two drachms of 

 the material used in the previous experiment were injected into the right femoral 

 vein. Kespiration ceased during the administration of the chloroform, but was 

 re-established after it had been carried on artificially for a few minutes. Towards 

 mid-day the dog died without having shown any choleraic symptoms, but having 

 apparently never recovered from the shock of the injection. 



Experiment XXVI. — A healthy dog was put under chloroform, and nearly half 

 an ounce of a choleraic evacuation which had been passed 12 days previously 

 injected into the median basilic vein. The operation was performed with hardly 

 any loss of blood, and the dog rapidly recovered from the influence of the 

 chloroform. 



It did not appear to suffer from the operation, and on the following day 

 appeared to be in perfect health. The fluid injected was swarming with bacteria 

 and vibriones. 



{i) — Choleraic material used being fifteen days old. 

 Experiment XXVII. — A large healthy pariah dog was put under the influence 



