I02 Researches Regarding Cholera : The Blood. [part i. 



The pleural cavities contained no fluid. The lungs were collapsed, airless and 

 bloodless. Both sides of the heart were full of blood. The blood contained active 

 monads and distinct bacteria. 



(^■) — Choleraic material used being eighteen days old. 



Experiment XXX. — A very young pup, similar to that employed in the 

 previous experiment, was put under chloroform, and nearly one drachm of the same 

 evacuation, which had now been kept for 18 days, was injected into the right femoral 

 vein. The operation was performed without loss of blood ; although respiration 

 ceased, it was readily re-established, the restoration being .apparently facilitated by 

 holding the animal up by the heels. There were no symptoms of intestinal affection, 

 but death supervened ten hours subsequent to the operation. 



{k) — Choleraic material being nineteen days old. 



Experiment XXXI. — A healthy dog of average size was put under the influence 

 of chloroform, and four drachms of the same evacuation employed in the previous 

 experiment were injected into the left femoral vein. There was no haemorrhage, nor 

 were the surrounding tissues disturbed. Towards the close of the operation the 

 respiration became imperfect, but it never fairly ceased, and the animal quickly 

 recovered from the effects of the chloroform. It did not appear to suffer from the 

 operation, and in two days appeared to be in perfect health. 



Experiment XXXII. — A healthy pup was put under the influence of chloroform, 

 and a few drachms of the supernatant fluid of an evacuation which had remained 

 for 19 days in the laboratory were injected into the right femoral vein. There was 

 hardly any haemorrhage during the operation, and the dog rapidly recovered from 

 the influence of the chloroform. It died five hours and a half afterwards, and a 

 'post-mortem examination was performed three hours after death. 



Rigor mortis was well marked. The abdomen was slightly swollen and there 

 was a little colourless fluid in the peritoneal cavity. The duodenum was of a pinkish 

 hue internally and contained thick, pale slimy matter. Further down, the contents 

 of the intestines were watery and of a sanguineous hue. There was pink coloration 

 throughout the jejunum and ileum, but both this and the fluidity of the contents 

 diminished in the neighbourhood of the ileo-coecal valve. The large intestine was 

 normal in appearance. The liver contained numerous light coloured patches similar 

 to those previously described in the post-mortem examination of Experiment XIII. 

 It was not congested. The spleen and kidneys were normal. 



The lungs were totally collapsed, airless and bloodless. The vence cavw, right 

 auricle, and right ventricle were full of dark and light coagula. The left cavities of 

 the heart contained a little dark coagulum. 



