PART I.] Injections of Aqueous Solutions into Veins of Animals. 103 



((/) — Choleraic material used being twenty-two days old. 



Experiment XXXIII. — A healthy dog was put under the influence of chloroform, 

 and half an ounce of the same choleraic dejection which was employed in Experiments 

 XXIII, etc., and which had now been kept for 22 days, was injected into the right 

 femoral vein. The operation was performed with perfect success ; the animal rapidly 

 recovered from the influence of the chloroform, did not appear in any way to suffer 

 from the injection, and three days subsequently was in a state of, seemingly, perfect 

 health. 



2. — Injections of aqueous solutions of choleraic material into the veins of animals. 

 (rt) — The solutions being recently prepared. 



Experiment XXXIV. — A young pariah pup was put under the influence of 

 chloroform at 8. a.m., and two drachms of a solution of choleraic material were injected 

 into the right femoral vein. The solution had been prepared about half an hour 

 before the operation, and consisted of equal measures of a perfectly fresh and filtered 

 choleraic evacuation, and of water. 



The animal rapidly recovered from the influence of the chloroform, and did 

 not appear to be much affected by the operation. Before evening he was as lively 

 as though nothing had happened to him, and was on the following morning made 

 the subject of Experiment XXXVIII, 



Experiment XXXV. — The pup which had two days previously been the subject 

 of Experiment V, and which appeared to be in perfect health, was put under chloroform, 

 and two drachms of a freshly prepared solution of choleraic evacuation were injected 

 into the left femoral vein. The solution consisted of the supernatant fluid and a 

 little sediment of an evacuation which had been kept for about 96 hours in the laboratory. 

 The animal rapidly recovered from the influence of the chloroform, was quite fresh 

 and lively shortly afterwards, and subsequently made its escape. 



Experiment XXXVI. — A healthy young pariah dog having been put under the in- 

 fluence of chloroform, half an ounce of an aqueous solution of choleraic material was injected 

 into the right femoral vein. The solution was perfectly fresh, and the evacuation 

 employed had been kept for 14 days. During the course of the day the dog 

 passed some watery reddish evacuations ; but, on the following morning, appeared 

 healthy, and continued to do so until the third day, when he was made the subject of 

 Experiment XL. 



Experiment XXXVII. — A healthy pariah pup was put under the influence of 

 chloroform, and a freshly prepared solution of choleraic material injected into the 



